Formalization of mouse embryo anatomy

Melatonin upregulates ErbB1 and ErbB4, two primary implantation receptors, in pre-implantation mouse embryos

Objective(s): To evaluated the effects of melatonin on early embryo competence and the expression rate of the primary implantation receptors (ErbB1 and ErbB4). Materials and Methods: Two-cell mouse embryos were cultured in 3 groups: simple media, melatonin-treated (10-9 M melatonin) and Luzindole-treated (10-9 M luzindole). Then, the rate of ErbB1 and ErbB4 gene and protein expression, the leve...

The Role of Glycoconjugates in Development of Floor Plate During Early Morphogenesis in Mouse Embryo

The floor plate is a small group of cells located at the ventral midline of neural tube. During early neurogenesis the floor plate plays critical role (s) in differentiation of ventral portion of neural tube.The purpose of this study was to determine the distribution of glycoconjugates in floor plate as well as underlying mesoderm (notochord) and their inductive activities in mouse embryos.Form...

Determination the Effects of Epidermal Growth Factor on Developmental Rates of Pre-Implantation Mouse Embryo

Purpose: To determine the effects of different doses of addition of exogenous epidermal growth factor (EGF) on development of pre-implantation mouse embryo. Materials and Methods: Mouse zygotes, two cell, 8 cell any morulae were collected from superovulated NMRI mice 24, 48, 64 and 80 hrs after hCG injection, respectively. The obtained embryos were cultured on medium alone, medium with 1, 4 an...

Mouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability

Potential of Mouse and Human Embryonic Fibroblasts to Support Mouse Embryo Development Following Exposure to Laboratory Temperature

Purpose: Present study was designed to evaluate the potential of co-culture systems to overcome deleterious effect of exposure of mouse 2-cell embryos to low temperature. Materials and Methods: 2-cell embryos were flushed from oviduct of super ovulated NMRI mice into HTF medium with 15% BSA. After washing 3 times with HBSS and with 15% BSA, embryos were exposed to laboratory temperature (LT) (...